Biotechnology 4313
Learning outcome
After completing the course, the students should have the following qualifications regarding knowledge, skills, and general competence:
- be familiar with many different applications of genetic engineering
- specialized insights in specific methods used in the field
- knowledge of ethical implications
- can use relevant methods in a manner that is required in a DNA lab.
- be able to apply techniques for extraction of DNA from various sources, saliva, blood, hair, tissue
- be able to optimize PCR reactions
- mastering primer design
- can carry out limited research projects under supervision for detection of various genetic differences
- planning to carry out tasks in line with ethical guidelines
- be able to convey specific topics in writing
Course Description
Key theme:
· extraction of DNA
· measurement of DNA concentration and purity
· PCR setup and optimization
· primer design
· detection of genetic variation using RFLP, microsatellites and AD-assay
· sequencing
· Real-time PCR
Ethical issues
Assessment Methods
Laboratory reports will constitute 40% and a final written examination (3 hours) represent 60% of the final grade.
Minor adjustments may occur during the academic year, subject to the decision of the Dean
Publisert av / forfatter Mona Sæbø <mona.saboSPAMFILTER@hit.no>, last modified Anette Norheim Fredly - 25/06/2012