Biotechnology 9002

Learning outcome

After completing the course, the students should have the following qualifications regarding knowledge, skills, and general competence:

  • be in the forefront of knowledge in regard to applications of genetic engineering
  • specialized insights in specific methods used in the field
  • be able to evaluate ethical implications
  • be able to carry out research in a manner that is required in a DNA lab. according to high international standards
  • be able to apply techniques for extraction of DNA from various sources, saliva, blood, hair, tissue
  • be able to optimize PCR reactions
  • mastering primer design
  • can handle complex research projects for detection of various genetic differences
  • be able to identify new relevant ethical issues
  • can communicate research according to international standards

Course Description

Key theme:

  • extraction of DNA
  • measurement of DNA concentration and purity
  • PCR setup and optimization
  • primer design
  • detection of genetic variation using RFLP, microsatellites and AD-assay
  • sequencing
  • Real-time PCR
  • Ethical issues

Assessment Methods

Laboratory reports (counts 40%) must be written individually and according to international standards. There is also a final written examination (3 hours) and counts for 60%. To pass the course the laboratory rapports and the examination must get the grade B or better.

Minor adjustments may occur during the academic year, subject to the decision of the Dean.

Minor adjustments may occur during the academic year, subject to the decision of the Dean

Publisert av / forfatter Mona Sæbø <mona.saboSPAMFILTER@hit.no>, last modified Anette Norheim Fredly - 13/09/2012