Applied Genetics 9002

Learning outcome

After completing the course, the students should have the following qualifications regarding knowledge, skills, and general competence:

  • be in the forefront of knowledge in regard to applications of genetic engineering
  • specialized insights in specific methods used in the field
  • be able to evaluate ethical implications
  • be able to carry out research in a manner that is required in a DNA lab. according to high international standards
  • be able to apply techniques for extraction of DNA from various sources, saliva, blood, hair, tissue
  • be able to optimize PCR reactions
  • mastering primer design
  • can handle complex research projects for detection of various genetic differences
  • be able to identify new relevant ethical issues
  • can communicate research according to international standards

Course Description

Key theme:

  • extraction of DNA
  • measurement of DNA concentration and purity
  • PCR setup and optimization
  • primer design
  • detection of genetic variation using RFLP, microsatellites and AD-assay
  • sequencing
  • Real-time PCR
  • Ethical issues

Teaching and Learning Methods

Some topics will be covered in lectures. The students must do all the laboratory work.

Assessment Methods

There is one final written examination (4 hours) represent 100% of the final grade.

  • Laboratory reports must be written individually and according to international standards, and are evaluated as approved/not approved.All laboratory reports must be approved before the final examination. To pass the course the laboratory rapports must get the grade B or better.
  • Its mandatory to attend all the lectures (80% to pass the course)

Minor adjustments may occur during the academic year, subject to the decision of the Dean

Publisert av / forfatter Mona Sæbø <mona.saboSPAMFILTER@hit.no> - 21/09/2015