Learning outcome

After completing the course, the students should have the following qualifications regarding knowledge, skills, and general competence:

• be familiar with many different applications of genetic engineering
• specialized insights in specific methods used in the field
• knowledge of ethical implications
• can use relevant methods in a manner that is required in a DNA lab.
• be able to apply techniques for extraction of DNA from various sources, saliva, blood, hair, tissue
• be able to optimize PCR reactions
• mastering primer design
• have knowledge of the development in the field of genetics and epigenetics
• can carry out limited research projects under supervision for detection of various genetic differences
• be able to convey specific topics in writing

Course Description

Key theme:

· extraction of DNA

· measurement of DNA concentration and purity

· PCR setup and optimization

· primer design

· detection of genetic variation using RFLP, microsatellites and AD-assay

· sequencing

· Real-time PCR

Ethical issues

Teaching and Learning Methods

Some of the topics will be covered in lectures. The students are also required to do independent studies of recent international articles in this field. The course is primarily a laboratory course and students must perform all the analysis.

Assessment Methods

Laboratory reports will constitute 40% and a final written examination (3 hours) represent 60% of the final grade.

Minor adjustments may occur during the academic year, subject to the decision of the Dean