Applied Genetics 9002

Læringsutbytte

After completing the course, the students should have the following qualifications regarding knowledge, skills, and general competence:

  • be in the forefront of knowledge in regard to applications of genetic engineering
  • specialized insights in specific methods used in the field
  • be able to evaluate ethical implications
  • be able to carry out research in a manner that is required in a DNA lab. according to high international standards
  • be able to apply techniques for extraction of DNA from various sources, saliva, blood, hair, tissue
  • be able to optimize PCR reactions
  • mastering primer design
  • can handle complex research projects for detection of various genetic differences
  • be able to identify new relevant ethical issues
  • can communicate research according to international standards

Innhold

Key theme:

  • extraction of DNA
  • measurement of DNA concentration and purity
  • PCR setup and optimization
  • primer design
  • detection of genetic variation using RFLP, microsatellites and AD-assay
  • sequencing
  • Real-time PCR
  • Ethical issues

Arbeids- og læringsformer

Some of the topics will be covered in lectures. The students are also required to do independent studies of recent international articles in this field. The course is primarily a laboratory course and students must perform all the analysis.

Vurderingsformer

There is one final written examination (4 hours) represent 100% of the final grade.

  • Laboratory reports must be written individually and according to international standards, and are evaluated as approved/not approved.All laboratory reports must be approved before the final examination. To pass the course the laboratory rapports must get the grade B or better.
  • Its mandatory to attend all the lectures (80% to pass the course)

Det tas forbehold om mindre justeringer i planen.

Publisert av / forfatter Mona Sæbø <mona.saboSPAMFILTER@hit.no> - 21.09.2015