Applied Genetics 9002
Læringsutbytte
After completing the course, the students should have the following qualifications regarding knowledge, skills, and general competence:
- be in the forefront of knowledge in regard to applications of genetic engineering
- specialized insights in specific methods used in the field
- be able to evaluate ethical implications
- be able to carry out research in a manner that is required in a DNA lab. according to high international standards
- be able to apply techniques for extraction of DNA from various sources, saliva, blood, hair, tissue
- be able to optimize PCR reactions
- mastering primer design
- can handle complex research projects for detection of various genetic differences
- be able to identify new relevant ethical issues
- can communicate research according to international standards
Innhold
Key theme:
- extraction of DNA
- measurement of DNA concentration and purity
- PCR setup and optimization
- primer design
- detection of genetic variation using RFLP, microsatellites and AD-assay
- sequencing
- Real-time PCR
- Ethical issues
Arbeids- og læringsformer
Some of the topics will be covered in lectures. The students are also required to do independent studies of recent international articles in this field. The course is primarily a laboratory course and students must perform all the analysis.
Vurderingsformer
There is one final written examination (4 hours) represent 100% of the final grade.
- Laboratory reports must be written individually and according to international standards, and are evaluated as approved/not approved.All laboratory reports must be approved before the final examination. To pass the course the laboratory rapports must get the grade B or better.
- Its mandatory to attend all the lectures (80% to pass the course)
Det tas forbehold om mindre justeringer i planen.
Publisert av / forfatter Mona Sæbø <mona.saboSPAMFILTER@hit.no> - 21.09.2015