Biotechnology 9002
Læringsutbytte
After completing the course, the students should have the following qualifications regarding knowledge, skills, and general competence:
- be in the forefront of knowledge in regard to applications of genetic engineering
- specialized insights in specific methods used in the field
- be able to evaluate ethical implications
- be able to carry out research in a manner that is required in a DNA lab. according to high international standards
- be able to apply techniques for extraction of DNA from various sources, saliva, blood, hair, tissue
- be able to optimize PCR reactions
- mastering primer design
- can handle complex research projects for detection of various genetic differences
- be able to identify new relevant ethical issues
- can communicate research according to international standards
Innhold
Key theme:
- extraction of DNA
- measurement of DNA concentration and purity
- PCR setup and optimization
- primer design
- detection of genetic variation using RFLP, microsatellites and AD-assay
- sequencing
- Real-time PCR
- Ethical issues
Arbeids- og læringsformer
Some of the topics will be covered in lectures. The students are also required to do independent studies of recent international articles in this field. The course is primarily a laboratory course and students must perform all the analysis.
Vurderingsformer
Laboratory reports (counts 40%) must be written individually and according to international standards. There is also a final written examination (3 hours) and counts for 60%. To pass the course the laboratory rapports and the examination must get the grade B or better.
Minor adjustments may occur during the academic year, subject to the decision of the Dean.
Det tas forbehold om mindre justeringer i planen.
Publisert av / forfatter Mona Sæbø <mona.saboSPAMFILTER@hit.no>, sist oppdatert av Anette Norheim Fredly - 13.09.2012